Aim of the present study was to develop chromatographic method of misoprostol epimer. The developed method was found to be simple, precise, accurate and reproducible. The linearity was in range of 500-3000 ng/spot, with correlation coefficient 0.998. Mean percentage recoveries were in the 99.54 - 100.01 range. Based on the best resolutions, we have selected the methanol: toluene (80:20 v/v) along with drop of acetic acid as a mobile phase. The rf value of epimer was in 0.47±0.02 range at wavelength 286 nm. The validation of proposed method was done as per ICH guidelines and was successfully applied to the estimation of Misoprostol. The proposed chromatographic method was simple and economic.
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